Influence of nutrient supplementation and stress conditions on the biomass and lipid production of Microchloropsis salina for biodiesel production

Abstract This work aimed to introduce a new technique for improving sustainable biodiesel from Microchloropsis salina by examining the impact of various nitrogen sources, salt stress, and carbon enrichment, individually or in combination, determine best treatment conditions biomass lipid productivity as well quality. M. effectively utilizes nitrate (NaNO 3 KNO ) organic (especially yeast extract glycine) but not ammonium growth. The highest were observed on (0.11 g L −1 day 52.11 μgmL , respectively). Lipid production enhanced 80% under 300 mM NaCl, while growth increased only 30%. Acetate could be used cultivate alga mixotrophically; glucose glycerol effective. ideal supply values 2 respectively. Nutrition promoted productivities 62% 95%, respectively, was reduced 16% combined 80%. All treatments significantly improved quality-related fatty acid profile.